中文Listeria monocytogenes PCR Detection Kit
Product Name
Listeria monocytogenes PCR Detection Kit
Size
48tests/kit, 50tests/kit
Intended Use
Listeria monocytogenes is a gram-positive microbacterium that can grow between 4℃ and 45℃. It is one of the main pathogens that threaten human health in refrigerated food. The main manifestations of infection are septicemia, meningitis and mononucleosis. This kit is suitable for the qualitative detection of Listeria monocytogenes in food, water samples, feces, vomit, bacterium-enhancing liquid and other samples using the principle of real-time fluorescence PCR.The kit is an ALL-READY PCR SYSTEM(Lyophilized), which contains the DNA amplification enzyme, reverse transcriptase, reaction buffer, specific primers and probes required for fluorescent PCR detection.
Storage & Shelf Life
(1)The kit can be transported at room temperature.
(2)The shelf life is 18 months at -20℃ and 12 months at 2℃~30℃.
(3)See the label on kit for the production date and expiration date.
(4)The lyophilized powder version reagent should be stored at -20℃ after dissolution and the repeated freeze -thaw should be less than 4 times.
Product Contents
| Components | Package | specification | Ingredient |
| Listeria monocytogenes PCR Mix | 1 ×bottle (Lyophilized powder) | 50Test | dNTPs, MgCl2, Primers, Probes,Reverse Transcriptase,Taq DNA polymerase |
| 6×0.2ml 8 well-strip tube(Lyophilized) | 48Test | ||
| Positive Control | 1*0.2ml tube (lyophilized) | 10Tests |
Plasmid containing Listeria monocytogenes specific fragments |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Instruments
GENECHECKER UF-150, UF-300 real-time fluorescence PCR instrument.
Operation Diagram
a)
b)
Pcr Amplification
Recommended Setting
| Step | Cycle | Temperature (℃) | Time | Fluorescence channel |
| 1 | 1 | 50 | 8min | |
| 2 | 1 | 95 | 2min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Collect FAM fluorescence |
*Note: The signals of FAM fluorescence channels will be collected at 60℃.
Interpreting Test Results
|
Channel |
Interpretation of results |
|
FAM Channel |
|
|
Ct≤35 |
Listeria monocytogenes Positive |
|
Undet |
Listeria monocytogenes Negative |
|
35<Ct≤40 |
Suspicious resut, retest* |
*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.
