中文Porcine reproductive and respiratory syndrome virus RT-PCR Detection Kit
Product Name
Porcine reproductive and respiratory syndrome virus RT-PCR detection kit(Lyophilized)
Size
48tests/kit, 50tests/kit
Intended Use
This kit uses real-time fluorescent RT-PCR method to detect the RNA of Porcine reproductive and respiratory syndrome virus nucleic acid detection kit(PRRSV) in tissue disease materials such as tonsils, lymph nodes and spleen and liquid disease materials such as vaccine and blood of pigs . It is suitable for the detection, diagnosis and epidemiological investigation of Porcine Blue Ear Virus. The kit is an ALL-READY PCR SYSTEM(Lyophilized), which contains the reverse transcriptase, DNA amplification enzyme, reaction buffer, specific primers and probes required for fluorescent RT-PCR detection.
Product Contents
| Components | Package | specification | Ingredient |
| PBEV PCR Mix | 1 ×bottle (Lyophilized powder) | 50Test | dNTPs, MgCl2, Primers, Probes,Reverse Transcriptase,Taq DNA polymerase |
| 6×0.2ml 8 well-strip tube(Lyophilized) | 48Test | ||
| Positive Control | 1*0.2ml tube (lyophilized) | 10Tests |
Plasmid or Pseudovirus containing PRRSV specific fragments |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Storage & Shelf Life
(1)The kit can be transported at room temperature.
(2)The shelf life is 18 months at -20℃ and 12 months at 2℃~30℃.
(3)See the label on kit for the production date and expiration date.
(4)The lyophilized powder version reagent should be stored at -20℃ after dissolution and the repeated freeze -thaw should be less than 4 times.
Instruments
GENECHECKER UF-150, UF-300 real-time fluorescence PCR instrument.
Operation Diagram
a) Bottle version:
b) 8 well-strip tube version:
Pcr Amplification
Recommended Setting
|
Step |
Cycle |
Temperature (℃) |
Time |
Fluorescence channel |
|
1 |
1 |
48 |
8min |
/ |
|
2 |
1 |
95 |
2min |
/ |
|
3 |
40 |
95 |
5s |
/ |
|
60 |
10s |
Collect FAM fluorescence |
*Note: The signals of FAM fluorescence channels will be collected at 60℃.
Interpreting Test Results
|
Channel |
Interpretation of results |
|
FAM Channel |
|
|
Ct≤35 |
Vibrio Parahaemolyticus Positive |
|
Undet |
Vibrio Parahaemolyticus Negative |
|
35<Ct≤40 |
Suspicious resut, retest* |
*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.
