中文Salmonella PCR Detection Kit
Product Name
Salmonella PCR Detection Kit (lyophilized)
Size
48tests/kit, 50tests/kit
Intended Use
Salmonella belongs to the Enterobacteriaceae and Gram-negative enterobacteria. Salmonella is a common food-borne pathogen and ranks first in bacterial food poisoning. The main symptoms of food poisoning caused by Salmonella are nausea, vomiting, abdominal pain, headache, chills and diarrhea. This kit uses the principle of real-time fluorescent PCR and is suitable for the qualitative detection of Salmonella in food, water samples, feces, vomit, and enrichment liquid.
Product Contents
| Components | Package | specification | Ingredient |
| PCR Mix | 1 ×bottle (Lyophilized powder) | 50Test | dNTPs, MgCl2, Primers, Probes,Taq DNA polymerase |
| 6×0.2ml 8 well-strip tube(Lyophilized) | 48Test | ||
| Positive Control | 1*0.2ml tube (lyophilized) | 10Tests |
Plasmid or Pseudovirus containing specific fragments |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 100uL | 0.9%NaCl |
Storage & Shelf Life
(1)The kit can be transported at room temperature.
(2)The shelf life is 18 months at -20℃ and 12 months at 2℃~30℃.
(3)See the label on kit for the production date and expiration date.
(4)The lyophilized powder version reagent should be stored at -20℃ after dissolution and the repeated freeze -thaw should be less than 4 times.
Instruments
GENECHECKER UF-150, UF-300 real-time fluorescence PCR instrument.
Operation Diagram
a) Bottle version:
b) 8 well-strip tube version:
Pcr Amplification
Recommended Setting
|
Step |
Cycle |
Temperature (℃) |
Time |
Fluorescence channel |
|
1 |
1 |
95 |
2min |
/ |
|
2 |
40 |
95 |
5s |
/ |
|
60 |
10s |
Collect FAM fluorescence |
Interpreting Test Results
|
Channel |
Interpretation of results |
|
FAM Channel |
|
|
Ct≤35 |
Salmonella Positive |
|
Undet |
Salmonella Negative |
|
35<Ct≤40 |
Suspicious resut, retest* |
*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.
