中文Shigella PCR Detection Kit
Product Name
Shigella PCR Detection Kit (lyophilized)
Size
48tests/kit, 50tests/kit
Intended Use
Shigella is a type of gram-negative brevis bacilli, belonging to intestinal pathogens, and the most common pathogen of human bacillary dysentery. This kit uses the principle of real-time fluorescent PCR, which is suitable for the determination of Shigella in food, water samples, feces, vomit, and enrichment liquid.
Product Contents
| Components | Package | specification | Ingredient |
| PCR Mix | 1 ×bottle (Lyophilized powder) | 50Test | dNTPs, MgCl2, Primers, Probes,Taq DNA polymerase |
| 6×0.2ml 8 well-strip tube(Lyophilized) | 48Test | ||
| Positive Control | 1*0.2ml tube (lyophilized) | 10Tests |
Plasmid or Pseudovirus containing specific fragments |
| Dissolving solution | 1.5 ml Cryotube | 500uL | / |
| Negative Control | 1.5 ml Cryotube | 100uL | 0.9%NaCl |
Storage & Shelf Life
(1)The kit can be transported at room temperature.
(2)The shelf life is 18 months at -20℃ and 12 months at 2℃~30℃.
(3)See the label on kit for the production date and expiration date.
(4)The lyophilized powder version reagent should be stored at -20℃ after dissolution and the repeated freeze -thaw should be less than 4 times.
Instruments
GENECHECKER UF-150, UF-300 real-time fluorescence PCR instrument.
Operation Diagram
a) Bottle version:
b) 8 well-strip tube version:
Pcr Amplification
Recommended Setting
|
Step |
Cycle |
Temperature (℃) |
Time |
Fluorescence channel |
|
1 |
1 |
95 |
2min |
/ |
|
2 |
40 |
95 |
5s |
/ |
|
60 |
10s |
Collect FAM fluorescence |
Interpreting Test Results
|
Channel |
Interpretation of results |
|
FAM Channel |
|
|
Ct≤35 |
Shigella Positive |
|
Undet |
Shigella Negative |
|
35<Ct≤40 |
Suspicious resut, retest* |
*If the retest result of FAM channel has a Ct value ≤40 and shows typical “S” shape amplification curve, the result is interpreted as positive, otherwise it is negative.
